Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-seq : 5 to 3 transcript coverage lnzzz Bioinformatics 7 01-07-2014 10:25 AM
Coverage, read length and variant calling accuracy ymc Bioinformatics 2 12-01-2013 02:22 PM
Transcript coverage estimates? tboothby General 2 12-14-2011 03:42 AM
Ion torrent blog on raw accuracy - good primer for accuracy grand challenge lek2k Ion Torrent 3 09-20-2011 01:13 AM

Thread Tools
Old 01-08-2014, 09:03 AM   #1
Senior Member
Location: .

Join Date: Mar 2011
Posts: 157
Default Coverage of a transcript and accuracy of DE

Hi all,

quite a basic question, but I find they can be the hardest to find answers for.

If I have transcripts with greater level of expression (measured by FPKM), can I have more confidence in the result if they are found differentially expressed? I am using edgeR under common dispersion model (with counts, not FPKM, to be clear; not ideal, I know, but at the end of the day we want genes potentially impacting a biological system in vivo and <100 appear when tagwise dispersion is used). Also I understand that 'more confidence' will be derived by using a stricter method, please humour me and my question

References to this end (either for or agin!) would be great.
bruce01 is offline   Reply With Quote

edger, rnaseq, transcript abundance

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:54 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO