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Old 09-14-2019, 03:35 PM   #21
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Location: Melbourne, Australia

Join Date: May 2019
Posts: 7

Thanks J for your comment.!

If you look carefully the size distribution you refer is there, just not well defined. 10x has indicated this is something they have observed also. This seems to happen when there is additional Mg in the tagmentation reaction.

Size selection could have been an issue but (re 74 bp fragment) but these never failed. Yet, I’ll definitely consider this.

On the other hand, the ambient DNA or nuclei desintegration during transition (not sure how that can happen, though) you mentioned would have generated a large peak at about 200 bp, re naked DNA. This is not the case, it shows basically no 200 bp peak and a large high molecular weight fragments. Nuclei prep is not the issue.

Thanks again for your helpful note!
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nextera sample prep, whole genome sequencing

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