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Old 04-21-2015, 02:34 PM   #1
Cyrus Taheri
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Location: Canada

Join Date: Aug 2014
Posts: 8
Question Miseq custom primer design, primer linker

Miseq custom primer design, primer linker

Many papers have used a 2bp primer linker in 5 end of the amplicon primers. As, I understand this is to prevent taxon specific PCR bias due to matches to the overhanger and/ or barcodes. I am wondering why 16S protocol did not add a linker.
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Old 04-21-2015, 06:25 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

Good question for Illumina. Addition of linker does make sense with no known or presumed detrimental effect.
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