Concentrating method for TruSeq lib prep in microRNA sequencing

candicechu · 01-20-2016, 01:17 PM

Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples.

I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method?

Any suggestions are appreciated. Thanks!

nucacidhunter · 01-20-2016, 05:40 PM

SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
After ChIP optimization the TruSeq LIB prep is not working anymore: peak at HMW!	EmiVig	Sample Prep / Library Generation	13	11-12-2014 02:54 AM
miRNA precipitation prior to Illumina TruSeq Small RNA lib prep	mdubs	Sample Prep / Library Generation	7	06-19-2013 08:24 AM
TruSeq lib prep anomaly	barturas	General	0	02-19-2013 12:47 PM
lib prep using SPRIworks	sgupta	Illumina/Solexa	12	03-09-2012 07:39 AM
SPRI lib prep?	BIG_SNP	Illumina/Solexa	32	03-23-2011 09:42 AM

01-20-2016, 01:17 PM	#1
candicechu Junior Member Location: College Station Join Date: Nov 2015 Posts: 3	Concentrating method for TruSeq lib prep in microRNA sequencing Hi all, We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine). We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples. I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method? Any suggestions are appreciated. Thanks!

01-20-2016, 05:40 PM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.