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Old 05-10-2016, 06:51 AM   #1
Mikemike107
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Default Sequence of Nextera transposon

Hi everyone,

it's could be interested for me to obtain the the entire Transposon sequence used in the NexteraŽ XT DNA Library Prep. Can anybody help me?

Thanks
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Old 05-20-2016, 08:49 AM   #2
pmiguel
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It is Tn5.

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Old 05-26-2016, 04:45 AM   #3
Mikemike107
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Thansk for your response. The entire 5000 bp sequence ?
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Old 05-26-2016, 08:47 AM   #4
pmiguel
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Wait. What do you mean by "transposon"? The protein part is pretty much Tn5, as I understand it, with maybe a couple of minor mutations. The DNA part is not, of course. The 5 kb DNA transposon is replaced with non-connected short sequences containing mainly the cis-regulatory sequence sufficient to cause recognition (binding) by the transposase.
Illumina provides part (or all?) of those sequences in the Illumina Adapter Sequence document:

http://support.illumina.com/download...ce-letter.html

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