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05-10-2016, 06:51 AM
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#1 |
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Junior Member
Location: France Join Date: May 2016
Posts: 2
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Sequence of Nextera transposon
Hi everyone,
it's could be interested for me to obtain the the entire Transposon sequence used in the NexteraŽ XT DNA Library Prep. Can anybody help me? Thanks |
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05-20-2016, 08:49 AM
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#2 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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It is Tn5.
-- Phillip |
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05-26-2016, 04:45 AM
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#3 |
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Junior Member
Location: France Join Date: May 2016
Posts: 2
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Thansk for your response. The entire 5000 bp sequence ?
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05-26-2016, 08:47 AM
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#4 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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Wait. What do you mean by "transposon"? The protein part is pretty much Tn5, as I understand it, with maybe a couple of minor mutations. The DNA part is not, of course. The 5 kb DNA transposon is replaced with non-connected short sequences containing mainly the cis-regulatory sequence sufficient to cause recognition (binding) by the transposase.
Illumina provides part (or all?) of those sequences in the Illumina Adapter Sequence document: http://support.illumina.com/download...ce-letter.html -- Phillip |
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