Changing index read length for Miseq run

WinterIsComing · 01-07-2019, 03:56 PM

Hi All, On the Miseq platform is it possible and relatively straightforward to perform a dual index read where each index read was 25 cycles rather than 6 or 8?

If it is possible to set a 25 cycle index read on Miseq, would data analysis be straightforward, or would special code need to be written to identify each index given its greater length?

Thanks.

thermophile · 01-08-2019, 01:31 PM

changing between 6 and 8 bp is just a matter of putting the index in the sheet. I'm not sure if you can do 25 bp index or if there's a limit either buried in the software. You could either call tech support or just try starting a run with 25bp index and see if it throws an error

WinterIsComing · 01-11-2019, 10:27 AM

Thanks. According to Illumina tech support many platforms have a cut off of 20, but miseq has no limit.

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01-07-2019, 03:56 PM	#1
WinterIsComing Junior Member Location: USA Join Date: Jan 2019 Posts: 3	Changing index read length for Miseq run Hi All, On the Miseq platform is it possible and relatively straightforward to perform a dual index read where each index read was 25 cycles rather than 6 or 8? If it is possible to set a 25 cycle index read on Miseq, would data analysis be straightforward, or would special code need to be written to identify each index given its greater length? Thanks.

01-08-2019, 01:31 PM	#2
thermophile Senior Member Location: CT Join Date: Apr 2015 Posts: 243	changing between 6 and 8 bp is just a matter of putting the index in the sheet. I'm not sure if you can do 25 bp index or if there's a limit either buried in the software. You could either call tech support or just try starting a run with 25bp index and see if it throws an error __________________ Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.

01-11-2019, 10:27 AM	#3
WinterIsComing Junior Member Location: USA Join Date: Jan 2019 Posts: 3	Thanks. According to Illumina tech support many platforms have a cut off of 20, but miseq has no limit.