Hi all,
My collaborator is interested in a region (about 8M bp long), that has been reported to susceptible to amplification/deletions.
Our goal is to perform sequencing experiments to specifically study the copy number variation of this region.
Whole-exome sequencing of course can help but most of the information will be wasted since our collaborator is not interested in other regions.
I wonder if we could capture the 8M region plus 3M up-stream and 3M down-stream region and then perform deep sequencing on the total 14M-bp region. Ideally, the up- and down-stream regions have no copy number change while it will be easy to quantify the copy numbers of the middle region.
I am not sure if the above strategy is feasible. Please help if you have a better idea of detecting copy number variation for a targeted region or if you have any advice for sequencing the above described region.
THanks a lot!
My collaborator is interested in a region (about 8M bp long), that has been reported to susceptible to amplification/deletions.
Our goal is to perform sequencing experiments to specifically study the copy number variation of this region.
Whole-exome sequencing of course can help but most of the information will be wasted since our collaborator is not interested in other regions.
I wonder if we could capture the 8M region plus 3M up-stream and 3M down-stream region and then perform deep sequencing on the total 14M-bp region. Ideally, the up- and down-stream regions have no copy number change while it will be easy to quantify the copy numbers of the middle region.
I am not sure if the above strategy is feasible. Please help if you have a better idea of detecting copy number variation for a targeted region or if you have any advice for sequencing the above described region.
THanks a lot!
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