Hello,
I'm seeing two size populations in my final RNAseq libraries, one at ~280 bp which is the expected library size and a larger one at ~700 bp which isn't expected.
I start with 500ng of intact total RNA and follow the TruSeq protocol with 15 cycles of PCR. When I back the number of cycles down to ten the larger sized population disappears (see picture).
Has anyone else experienced this?
Thanks!
I'm seeing two size populations in my final RNAseq libraries, one at ~280 bp which is the expected library size and a larger one at ~700 bp which isn't expected.
I start with 500ng of intact total RNA and follow the TruSeq protocol with 15 cycles of PCR. When I back the number of cycles down to ten the larger sized population disappears (see picture).
Has anyone else experienced this?
Thanks!
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