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Old 02-18-2014, 05:45 AM   #1
xfh
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Location: China

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Post how to make ANCOVA in two indenpendent dataset

for example, there are two gene expression microarray datasets,

samples 1
gene 14 year-old 25 year-old .....

LDH 0.2 0.8 ....

samples 2
gene 12 year-old 20year-old .....

LDH 0.25 0.78

if i know the LDH gene expression level is increasing with age, I want to know the correlataion of the gene expression change in aging process between two samples, how i calculated the Pearson correlation. and how I make a ANCOVA in the above LDH expression between the two samples.
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Old 02-18-2014, 06:19 AM   #2
JackieBadger
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Two decent introductions into ANCOVA

http://r-eco-evo.blogspot.ca/2011/08...slopes-by.html

http://www.stat.columbia.edu/~martin/W2024/R8.pdf
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Old 02-18-2014, 04:18 PM   #3
xfh
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thank you for your information. and here i want to ask how to handle the dataset. because the age and number of samples are different. the data is not paired. like ancova(x,y), the vector x,y should be same length and paired, right?
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Old 02-18-2014, 04:39 PM   #4
JackieBadger
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Hmmm good question. I think someone with a stats background (i.e. not me) could answer your question accurately, but I'll have a guess:

I think you would do two separate ANCOVAs, one each for you two age brackets. From these I believe you could compare the correlation p values, but not the slopes i.e. the effect size. For this I think you would need to use something like as Tukey's ad hoc test.

Don't hold me to that!
You should get advice from someone with more knowledge on the matter than I can provide.

J
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Old 02-18-2014, 05:04 PM   #5
xfh
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thank you very much again, someone told me that the dataset can be fitting with R package "spline.smooth" first and then make ANCOVA ~
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