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  • Simulate reads from circular ref

    Hi,

    Does anybody have a method for simulating reads from a reference? I would like the read simulator to be able to treat the reference as circular.

    I have been using this script http://socwiki.wordpress.com/2011/05...ngss-sequence/ but it has been returning libraries with odd insert sizes (not sure how to fix it). I have also tried using one of my own python scripts but it's not good enough for the job.

    Appreciate any input!

    Thanks
    S

  • #2
    Download Grinder for free. Grinder is a versatile open-source bioinformatic tool to create simulated omic shotgun and amplicon sequence libraries for all main sequencing platforms.

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    • #3
      Hi, that looks great but I can't see an option to treat the input sequences as circular. Am I missing something? If so could you point me in the right direction?


      Thanks
      S

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