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  • rnacocktail basic questions

    My lab typically does RNA-seq ~once a year and I do the bioinformatic analysis myself. So the annoying part for me being not the expert in routine NGS analysis from raw data, every time I get new RNA-seq raw data I almost have to redo the setup and software installation with some new (and maybe better) analysis tools developed and my working computer (MacOS) being upgraded.

    I have some basic concept of using Unix but yet I feel the most challenging part
    to me to to get these new softwares running correctly.

    This time, I decided to try out RNACocktail based on a Nature Communications paper.

    After some trial and error, I managed to get it installed on my Mac (MacOS 10.13).

    "pip install rnacocktail-0.2.1.tar.gz --user
    Processing ./rnacocktail-0.2.1.tar.gz
    Collecting pysam (from RNACocktail-Pipeline==0.2.1)
    Using cached pysam-0.12.0.1.tar.gz
    Collecting pybedtools (from RNACocktail-Pipeline==0.2.1)
    Using cached pybedtools-0.7.10.tar.gz
    Requirement already satisfied: six in /System/Library/Frameworks/Python.framework/Versions/2.7/Extras/lib/python (from pybedtools->RNACocktail-Pipeline==0.2.1)
    Installing collected packages: pysam, pybedtools, RNACocktail-Pipeline
    Running setup.py install for pysam ... done
    Running setup.py install for pybedtools ... done
    Running setup.py install for RNACocktail-Pipeline ... done
    Successfully installed RNACocktail-Pipeline-0.2.1 pybedtools-0.7.10 pysam-0.12.0.1"

    However, after I tried this:
    run_rnacocktail.py -h

    I got this error message:

    -bash: run_rnacocktail.py: command not found

    Any suggestions? Thanks.
    Last edited by neokao; 09-28-2017, 07:10 PM.

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