Hi,
I have done the De novo RNA-Seq Assembly using Trinity with my fish data, but the number of unigenes I get is 333,843, and the N50 Length is 396. I think the number of unigenes is too many. So which parameter should I set to reduce the number of unigenes and make the N50 length longer?
My trinity version is: Trinityrnaseq_r20131110
All of the parameter is default.
Thanks a lot for any suggesstion!
I have done the De novo RNA-Seq Assembly using Trinity with my fish data, but the number of unigenes I get is 333,843, and the N50 Length is 396. I think the number of unigenes is too many. So which parameter should I set to reduce the number of unigenes and make the N50 length longer?
My trinity version is: Trinityrnaseq_r20131110
All of the parameter is default.
Thanks a lot for any suggesstion!
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