TruSeq PCR Prep Box - DNA/RNA

[MLog]

Hi all,

I wonder is there any difference between TruSeq DNA PCR Prep Box and TruSeq DNA PCR Prep Box?
Looks like we accidentally used RNA box instead of DNA for enrichment of DNA libraries. It worked well but now I'll have to use DNA box for RNA libraries because I don't have a new RNA prep kit at this moment. Will this work? Probably, some modifications should be done? (I know that DNA adapters have higher concentration than RNA ones, so I guess that primers from DNA PCR box can have higher concentration too.)

Thanks!
Maria.

[amazonic9]

Hi!

I guess it should work, as we have received Truseq RNA kits with the Truseq DNA PCR box... so, either Illumina had a problem with the shipment and we received something we were not supposed to get, or both truseq RNA and DNA PCR boxes are the same (although I would expect a difference in primer concentration, as you said).

Best,

amazonic9

[Lanlan]

Yes, we also received Truseq RNA kits with the DNA PCR box....

Hi!

I guess it should work, as we have received Truseq RNA kits with the Truseq DNA PCR box... so, either Illumina had a problem with the shipment and we received something we were not supposed to get, or both truseq RNA and DNA PCR boxes are the same (although I would expect a difference in primer concentration, as you said).

Best,

amazonic9

[MLog]

Thanks for sharing your ideas and experience. I ended up with doing no modifications and this worked pretty well. I was afraid that I could have a lot of adapter dimers but this does not seem to be the case.