Different read length sequencing datasets

g781 · 07-07-2010, 12:40 AM

Hi all,

I have two Illumina solexa sequencing dataset, one of the read lengths is 35bp, other is 75bp. Whether the different read length sequencing datasets may effect the analyzed results.

mgogol · 07-07-2010, 08:20 AM

If it does, you could always trim the 75 base reads to 35 using fastx and realign.

g781 · 07-07-2010, 09:45 AM

I see, thank for your helping.

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07-07-2010, 12:40 AM	#1
g781 Member Location: Taiwan Join Date: May 2010 Posts: 25	Different read length sequencing datasets Hi all, I have two Illumina solexa sequencing dataset, one of the read lengths is 35bp, other is 75bp. Whether the different read length sequencing datasets may effect the analyzed results.

07-07-2010, 08:20 AM	#2
mgogol Senior Member Location: Kansas City Join Date: Mar 2008 Posts: 197	If it does, you could always trim the 75 base reads to 35 using fastx and realign.

07-07-2010, 09:45 AM	#3
g781 Member Location: Taiwan Join Date: May 2010 Posts: 25	I see, thank for your helping.