chord->getLength Newbler error

veena · 02-11-2009, 12:49 PM

Hi,

I'm trying to run a Newbler assembly on a sample that is predicted to be ~8-9Mb but get the following error message:

Error: An internal error has occurred in the computation.
Assertion: chord->getLength() > 10
Location: 'bool ChordMatrix::checkAddChordColumn(Chord*, int)'
[ChordMatrix.cpp:182]
Please report this error to your customer support representative.

I was wondering if this rings a bell to anybody and if you knew how to fix it.

Thanks!

Torst · 02-18-2009, 03:15 PM

I experienced this error a lot when using FASTA file input rather than SFF files. This was with Version 1 of Newbler. If you can get Version 2 of Newbler, try that.

plattsa · 07-23-2010, 10:32 AM

I still get this error with FASTA files in 2.3, no real help from Roche when I contacted them.

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02-11-2009, 12:49 PM	#1
veena Junior Member Location: baltimore Join Date: Feb 2009 Posts: 9	chord->getLength Newbler error Hi, I'm trying to run a Newbler assembly on a sample that is predicted to be ~8-9Mb but get the following error message: Error: An internal error has occurred in the computation. Assertion: chord->getLength() > 10 Location: 'bool ChordMatrix::checkAddChordColumn(Chord*, int)' [ChordMatrix.cpp:182] Please report this error to your customer support representative. I was wondering if this rings a bell to anybody and if you knew how to fix it. Thanks!

02-18-2009, 03:15 PM	#2
Torst Senior Member Location: The University of Melbourne, AUSTRALIA Join Date: Apr 2008 Posts: 275	I experienced this error a lot when using FASTA file input rather than SFF files. This was with Version 1 of Newbler. If you can get Version 2 of Newbler, try that.

07-23-2010, 10:32 AM	#3
plattsa Member Location: Michigan Join Date: Mar 2009 Posts: 17	I still get this error with FASTA files in 2.3, no real help from Roche when I contacted them.