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Old 12-15-2015, 10:43 AM   #1
Junior Member
Location: Chicago

Join Date: Dec 2015
Posts: 1
Default Seq of PCR product with great seq but one peak very low intensity


I have not encountered anything like this before so I'm hoping someone could shed some light. I have a beautiful sequence for a PCR product and have noticed that although the rest of the sequence has uniform intensity, I have one peak that is so drastically diminished in its intensity that the software calls it a deletion, however, upon closer inspection the peak is very very small. What could cause this? Thanks!
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Old 12-16-2015, 06:58 AM   #2
MU Core
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 57

Sharones, would you be able to post the image of the peak trace?
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