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03-22-2016, 06:32 AM
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#1 |
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Junior Member
Location: The Netherlands Join Date: Mar 2016
Posts: 6
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epiGBS
Nature methods epiGBS article:
Please notice our Nature methods epiGBS article on epiGBS: epiGBS: reference-free reduced representation bisulfite sequencing doi:10.1038/nmeth.3763 I made this thread to help get you information about new updates on the protocols (new enzyme combinations, adapter innovations) and to get in contact for help or collaborations. Our great protocol will really help you to steam up your DNA genotyping and methylation research on big amount of samples for affordable costs. Niels Wagemaker |
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10-27-2016, 12:10 AM
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#2 |
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Senior Member
Location: Leipzig, Germany Join Date: May 2012
Posts: 275
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DNA Methylation Data Analysis Workshop
How to use bisulfite-treated sequencing to study DNA methylation When? 22. - 25. November 2016 Where? Leipzig, Germany Link? http://www.ecseq.com/workshops/works...-Data-Analysis
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ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS). |
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10-27-2016, 04:45 AM
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#3 |
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Junior Member
Location: The Netherlands Join Date: Mar 2016
Posts: 6
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Epigbs enzyme combinations
Next to the published enzyme combinations we have available:
Improved solutions for Smal genomes: (arabidopsis): csp6I * NsiI Average genomes: (scabiosa columbaria): AseI * NsiI Large genomes: (sequoia): PacI *NsiI All with methylation insensitive enzymen. PacI needs different adapter set as other 2 enzyme combi's More info: mail me |
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| Tags |
| epigbs, gbs, methylation, smp, snp |
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