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01-26-2018, 12:39 PM
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Junior Member
Location: montevideo Join Date: Oct 2016
Posts: 1
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DESEQ2 Desing formula time series experiments with sham controls.
Hi. I have a time series RNASEQ experiment with 2 controls and 3 treated samples for each time point.
sample time condition A 1 control B 1 control C. 1 treated D. 1 treated E 1 treated F 2 control G 2 control H 2 treated I 2 treated J 2 treated K 3 control L 3 control M 3 treated N 3 treated O 3 treated I want to obtain the Differential gene expression for each of the time points and between time points: time_1_control_vs_treated, time_2_control_vs_treated, time_3_control_vs_treated. time1_vs_time2 time2_vs_time3 time1_vs_time3 What should I include in the design formula? Thank you |
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| Tags |
| deseq2, dge, expression, rnaseq, sequencing |
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