Hello, we use Kapa qPCR kits to quantify our libraries before sequencing. Recently, the NTC wells are giving low cq values which means there are contaminants and therefore the data is not reliable. Despite cleaning the wells, changing kits, and re-aliquoting reagents, the NTC wells are still giving the same result.
Has anyone else had this issue? Does anyone have any suggestions?
Thanks in advance.
Has anyone else had this issue? Does anyone have any suggestions?
Thanks in advance.
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