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I want to do chip-seq for a transcription factor in bacteria for Illumina. I' need help for sample preparation for bacteria since all I find is eukaryotes. Brand new with the technique, sorry!
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I haven't done ChIP in bacteria but once you have the ChIP material I would think making the libraries would be the same. I'm waiting to get back sequencing data from my first ChIP seq libraries. If all looks good then I will be able to give some advice. You could contact Illumina to see if they have any protocols for ChIP in prokayotes. -
A very interesting study shows some of the pitfalls of ChIP applications and offers some advances to published protocols.
The paper is dedicated to ChIP on Chip but most of it also applies to Chip-Seq, too.
If you're new to the tech, I would recommend reading this paper.
Here's the ref: Waldminghaus & Skarstad 2010, BMC Genomics, 11:414Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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