Hi all,
This is probably a really dumb question but I was just wondering if someone could explain to me how adaptor sequences affect RNA seq data.
I've carried out some sequence alignments on my samples without trimming or removing them & the majority of reads(over 85%) are aligning....is this normal? would it improve if I removed them or do I need to?
Thanks in advance for advice!!
This is probably a really dumb question but I was just wondering if someone could explain to me how adaptor sequences affect RNA seq data.
I've carried out some sequence alignments on my samples without trimming or removing them & the majority of reads(over 85%) are aligning....is this normal? would it improve if I removed them or do I need to?
Thanks in advance for advice!!
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