Hi,
I used to standalone BLAST to subtract the Clostridium difficile proteome from the human proteome, using the command
>blastp -db Human -in Mybacteria.fas -out results.fas -evalue 1e-3 -outfmt 4
The results showed the query hits where the Clostridiums proteome matched the humans, I now need to subtract these matched sequences from the whole clostridium proteome and wondered if there was a quicker efficient way of doing this.
Also if there was is a website that showed how to analyse the results.
Many thanks,
Dani
I used to standalone BLAST to subtract the Clostridium difficile proteome from the human proteome, using the command
>blastp -db Human -in Mybacteria.fas -out results.fas -evalue 1e-3 -outfmt 4
The results showed the query hits where the Clostridiums proteome matched the humans, I now need to subtract these matched sequences from the whole clostridium proteome and wondered if there was a quicker efficient way of doing this.
Also if there was is a website that showed how to analyse the results.
Many thanks,
Dani
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