Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • High-Throughput DNA Shearing

    Hello,


    Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously? I know there are some systems out there that can sonicate a whole plate. Has anybody tried them? Also, I have seen several posts on the Bioruptor: does anybody know if it can go down to 150-200bp peak size? How long does it take?

    Thank you so much!
    Last edited by ABSOLiDuser; 03-09-2009, 07:35 PM.

  • #2
    We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?

    Comment


    • #3
      Thanks EPI.
      Last edited by ABSOLiDuser; 03-09-2009, 07:34 PM.

      Comment


      • #4
        Piranha

        Hi,

        I believe ABI is working on a 96 well sonicator for SOLiD called a 'Piranha'. I heard something about it when I attended a SOLiD V3 launch seminar. Talk to your rep and see if this is the case.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM
        • seqadmin
          Techniques and Challenges in Conservation Genomics
          by seqadmin



          The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

          Avian Conservation
          Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
          03-08-2024, 10:41 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 06:37 PM
        0 responses
        11 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, Yesterday, 06:07 PM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-22-2024, 10:03 AM
        0 responses
        51 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-21-2024, 07:32 AM
        0 responses
        67 views
        0 likes
        Last Post seqadmin  
        Working...
        X