Hi,
I am looking for a simple PCR based assay to evaluate if there is TruSeq amplified library present in our Pre-PCR lab. I would like to use the Bioanalyzer to quantitate. Would something as simple as designing primers to the P5/P7 and amplifying work? Could I just use the leftover primers from the library prep kit? How many cycles would you recommend? Any input or ideas welcomed.
I am looking for a simple PCR based assay to evaluate if there is TruSeq amplified library present in our Pre-PCR lab. I would like to use the Bioanalyzer to quantitate. Would something as simple as designing primers to the P5/P7 and amplifying work? Could I just use the leftover primers from the library prep kit? How many cycles would you recommend? Any input or ideas welcomed.
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