Howdy all,
Wondering if anyone has had any success running "half reactions" of NexteraXT libraries or otherwise stretching those reagents? At the end of the library cleanup, if memory serves you only carry a little more than half of the reaction into bead based balancing. The bulk of our sequencing cost is in the actual library preparation steps, so cutting that in half is a tempting experiment but it would involve pipetting some very small volumes (0.5 ul). Does anyone have any experience with trying this?
Best,
docbio
Wondering if anyone has had any success running "half reactions" of NexteraXT libraries or otherwise stretching those reagents? At the end of the library cleanup, if memory serves you only carry a little more than half of the reaction into bead based balancing. The bulk of our sequencing cost is in the actual library preparation steps, so cutting that in half is a tempting experiment but it would involve pipetting some very small volumes (0.5 ul). Does anyone have any experience with trying this?
Best,
docbio
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