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  • Clontech kit or illumina kit?

    Hi, do you guys have some experience with single cell RNA seq with Clontech Smarter single cell rna kit? I have just entered this area of study. The clontech guide says that there are 2 ways of library construction after the cDNA synthesis: a. Nextera XP DNA Kit (illumina) b. fragmentation with ultrasound following Clontech sample preparation kit. I'm really confused cos I can't find any reference. Are you using anyone of them or have you compared theses two? Thank you so much!

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  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
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    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

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