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Old 10-18-2017, 05:00 AM   #1
ecSeq Bioinformatics
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Location: Leipzig, Germany

Join Date: May 2012
Posts: 206
Lightbulb FINAL CALL: Epigenetics Workshop - DNA Methylation Data Analysis

DNA Methylation Data Analysis
How to use bisulfite-treated sequencing to study DNA methylation

Link to workshop page

When?
29. November - 1. December 2017

Where?
PC-College, Stresemannstraße 78, 10963 Berlin, Germany


Scope and Topics
The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:
  • be familiar with the sequencing method of Illumina
  • understand how bisulfite sequencing works
  • be aware of the mapping problem of bisulfite-treated data
  • understand how bisulfite-treated reads are mapped to a reference genome
  • be familiar with common data formats and standards
  • know relevant tools for data processing
  • automate tasks with shell scripting to create reusable data pipelines
  • perform basic analyses (call methylated regions, perform basic downstream analyses)
  • plot and visualize results (ready-to-publish)
  • be able to reuse all analyses

Target Audience
  • biologists or data analysts with no or little experience in analyzing bisulfite sequencing data

Requirements
  • basic understanding of molecular biology (DNA, RNA, gene expression, PCR, ...)
  • the data analysis will partly take place on the linux commandline. Is is therefore beneficial to be familiar with the commandline and in particular the commands covered in the Learning the Shell Tutorial

Included in the Course
  • Course materials
  • Catering
  • Conference Dinner

Trainers
  • Dr. Helene Kretzmer (Max Planck Institut for Molecular Genetics) is working on DNA methylation analyses using high-throughput sequencing since 2011. She was responsible for the bioinformatic analysis of MMML-Seq study of the International Cancer Genome Consortium (ICGC).
  • Dr. Christian Otto (ecSeq Bioinformatics GmbH) is one of the developers of the bisulfite read mapping tool segemehl and is an expert on implementing efficient algorithms for HTS data analyses.

Key Dates
Closing Date of Registration: 01 November 2017
Workshop: 29 November - 1 December 2017 (9 am - 5 pm)

Attendance
Location: PC-College, Stresemannstraße 78, 10963 Berlin, Germany
Language: English
Available seats: 25 (first-come, first-served)

Registration fees:

989 EUR (without VAT)

Travel expenses and accommodation are not covered by the registration fee.

Contact
ecSeq Bioinformatics GmbH
Sternwartenstr. 29
04103 Leipzig
Germany
Email: [email protected]

Visit: http://www.ecseq.com/workshops/works...-Data-Analysis
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ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).
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