Hi all!
If:
- all the paramethers about the initial DNA are OK (quantity, 260/280, 260/230 and integrity)
- Bioanalyzer results are OK
- library post OT2 is OK
- run results are OK (% loading wells, low policlonality, a lot of MB, size reads, etc)
Is possible that the low data quality has relationship with the "library prep" or is just a PGM problem?
(this situation is just in one case, the other data runs are OK)
Thanks in advance!
If:
- all the paramethers about the initial DNA are OK (quantity, 260/280, 260/230 and integrity)
- Bioanalyzer results are OK
- library post OT2 is OK
- run results are OK (% loading wells, low policlonality, a lot of MB, size reads, etc)
Is possible that the low data quality has relationship with the "library prep" or is just a PGM problem?
(this situation is just in one case, the other data runs are OK)
Thanks in advance!