Don't need any help, just curious. As I understand it Illumina make their flowcells with an excess of primers bound to the slide. It's the up to whoever loads the cBot/clusterstation to work out how much library to add. This is quite a delicate business as everyone wants the highest read numbers possible, but if you overload then the clusters overlap and you loose a lot of the data. Would it be easier for Illumina to make the flowcells with the primers already attached at the optimum density. Then the operator can add an excess of library happy in the knowledge that optimal cluster density can be achieved. If they were feeling really flash then they could somehow print the primers on the slide in an ordered pattern so that the computer knows where to look for clusters, and thus pack them in even tighter.
I'm probably missing something really obvious. Maybe someone can enlighten me.
Cheers.
I'm probably missing something really obvious. Maybe someone can enlighten me.
Cheers.
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