Hello, I am comparing transcription in KO and WT ES cell lines and am basically worried about pseudoreplication.
If a single cell line is split into two, and then they undergo many passages separated from each other, would that qualify as "having undergone independent growth"?
Would they then be considered independent biological replicates?
Many thanks
Alex
If a single cell line is split into two, and then they undergo many passages separated from each other, would that qualify as "having undergone independent growth"?
Would they then be considered independent biological replicates?
Many thanks
Alex
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