I am interested in directionally sequencing mRNA from bacteria (who do not have a polyA tail). Since Illumina's Ribo-zero kit depletes only mammalian rRNA I will not be able to use it. Therefore, I want to use my own bacterial Ribo-Zero kit and then use the Illumina stranded (directional) mRNA sequencing kit.
The main difference I see between the Ribo-Zero total RNA stranded kit and the mRNA stranded kit (apart from the mRNA/total RNA isolation procedure) is in the buffers used for fragmentation (FPF buffer for mRNA, EFP buffer for total RNA). Can the FPF buffer of the directional mRNA seq kit be used for fragmentation in solution rather than on the beads?
The main difference I see between the Ribo-Zero total RNA stranded kit and the mRNA stranded kit (apart from the mRNA/total RNA isolation procedure) is in the buffers used for fragmentation (FPF buffer for mRNA, EFP buffer for total RNA). Can the FPF buffer of the directional mRNA seq kit be used for fragmentation in solution rather than on the beads?
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