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Hi, I have noticed in HG19, there are sometimes a segment of sequence that are in lower case? Does this have a special meaning?
Thanks
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Often, sequence in loweracse is repetative. It's been soft masked so you can distinguish it from unique sequence -
What about those characters masked with "N", I thought those mean repeats too?
BTW how does aligner like BWA take care "N"s, if it sees Ns in the reference does it automatically ignore those segment?Comment
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Can you kindly point out which paper it is please?Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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