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Old 08-09-2017, 10:19 PM   #1
hyssakura
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Location: Shanghai

Join Date: Mar 2012
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Question Which strand of dsDNA library is the initial template on the Flow cell

The both of complement ssDNA can hybirdize to p5 or p7 oligos on flow cell.
In this case, the sense strand and the antisense strand will form two identical clusters after bridge PCR. So the duplication rate will be 50%.

But in the skematic of Illumina, only p7 oligos can extand to be the initial template before bridge PCR. It means only one strand of dsDNA can hybirdize to P7 oligos.

So Why p5 can't hybirdize one strand of dsDNA? Are p5 oligos is blocked?
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