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Old 07-22-2017, 05:37 AM   #1
guardian_priest
Junior Member
 
Location: Taiwan

Join Date: Oct 2013
Posts: 1
Unhappy 12 mer for Miseq custom sequencing primer

Hi~
Does anyone had read the article below?

Treseder et al., 2016
http://onlinelibrary.wiley.com/doi/1...C13B9AE.f03t04

According to the Material and Methods (attachment), they wanted to use stagger strategy from (Tremblay et al., 2015) but the PAD region has only 12bp, I used custom Index primer and custom R2 sequencing primer based on PAD sequence for a Miseq sequencing run but got a fail run when index run had started. I think the 12mer Index primer might be the problem. Here's me custom primer

Index sequencing primer
GGCTGACTGACT
Read 2 sequencing primer
AGTCAGTCAGCC

Can anyone help me for this issue?
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File Type: png 未命名-2.png (42.9 KB, 6 views)
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