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Our lab has built a drop-seq setup. Going over the protocol, I see that the cells are lysed in a solution of sarkosyl, Ficoll-400, tris, EDTA, and DTT. I'm not intimately familiar with the biochemstry of this lysis buffer. I'm wondering if it is effective at inactivating endogenous RNAses? I haven't found anyone online that has discussed degradation of RNA at this stage. But the pumping can take quite a while, depending on the scale of the experiment (at least 15 minutes per sample). I am just curious if this is something I should or shouldn't be concerned about.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM