I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant but I am not sure on the specifics. Can anyone help with this? Specifically: -
- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?
- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?
- Why does my ribosomal RNA end up bound to beads but the rest doesn't?
- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?
- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?
- Why does my ribosomal RNA end up bound to beads but the rest doesn't?
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