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  • Compiling reads from lanes

    Dear members,

    We have done some RNA-seq on Illumina. Our samples were pooled and run on 3 lanes. So now I have 3 files with reads for each sample. I would like to put together these 3 files to a single one so I can upload them on galaxy and use the analysis tools available there, but I don't know how to . I have no programming skills so I wouldn't have a clue where to start from.
    What do I do?

    Thanks!!!

  • #2
    Are you on linux? You can just use the "cat" command, like so:

    cat filename1 filename2 filename3 > newfilename

    Does this help?

    Comment


    • #3
      Many thanks

      Originally posted by genlyai View Post
      Are you on linux? You can just use the "cat" command, like so:

      cat filename1 filename2 filename3 > newfilename

      Does this help?
      I have access to Linux as well and this did help a lot . Many many thanks!!!

      Comment

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