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Old 07-26-2013, 01:47 PM   #1
qingchang
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Default WGA techniques

I am new in NGS. I am wondering if anybody knows the difference between 2 WGA methods:

MALBAC of Sunney Xie

vs

Rubicon's PicoPlex

I thought they are similar, but I could be wrong.
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Old 08-17-2013, 05:56 PM   #2
Genohub
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Default WGA, MALBAC and Rubicon

Our blog post describes several single cell sequencing approaches. For more details check out their patents:

Rubicon

MALBAC
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Old 04-06-2018, 01:50 AM   #3
raimotanzi
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Default

They are all good when you have "few" cells, like 10 or more, but all suffer when you have a "single" cell. Mostly you will experience high allelic dropout and high noise in Copy number profiles. Currently, the method which is reported by multiple comparative articles to be the best performer for "single" cell WGA is Ampli1 http://www.siliconbiosystems.com/ampli1-wga-kit
PLOS ONE | https://doi.org/10.1371/journal.pone.0193689 March 1, 2018
PLOS ONE | DOI:10.1371/journal.pone.0171566 February 16, 2017
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