dUTP/UDG or RNA ligation sequencing method?

[bernardo_bello]

Hi community,

I would like to know the strand specificity type of RNA libraries prepared with Ion Total RNA-Seq Kit v2 - Life Technologies protocol.

Thank you.

Regards, Bernardo

[GenoMax]

See if this has what you need: http://tools.invitrogen.com/content/...s/4476286B.pdf

[bernardo_bello]

See if this has what you need: http://tools.invitrogen.com/content/...s/4476286B.pdf

Thank you GenoMax,

Which is the difference between dUTP/UDG and RNA ligation sequencing method?

Bernardo

[snetmcom]

One converts to cDNA and digests away a strand to maintain directional infomration.

The Ion kit ligates dsAdapters directly to RNA, which typically has better results.

[bernardo_bello]

One converts to cDNA and digests away a strand to maintain directional infomration.

The Ion kit ligates dsAdapters directly to RNA, which typically has better results.

Thank you snetmcom,

Now it's clear.

I found in this paper the dUTP/UDG method explained, althoght the Ion Torrent RNA-seq library preparation is based on ligation method.

http://www.ncbi.nlm.nih.gov/pubmed/21943893