Can we sequence RNA directly

SDPA_Pet · 04-06-2017, 05:03 PM

Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now?

Or now most sequencing center still use cDNA to do metatranscriptomics.

nucacidhunter · 04-06-2017, 09:59 PM

I know two technologies for direct transcriptome analysis:
1- nanoString: it can only quantify known transcripts
2- MinIon: the kit currently is offered for early access

cDNA still is the most common method.

Bukowski · 04-07-2017, 05:00 AM

http://biorxiv.org/content/early/2016/08/12/068809

gringer · 04-07-2017, 05:17 AM

Quote:

Originally Posted by nucacidhunter

1- nanoString: it can only quantify known transcripts

nanoString doesn't "sequence" the RNA, unless you're referring to Hyb & Seq (which can sequence novel sequences, but is still in the technology development stages).

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04-06-2017, 05:03 PM	#1
SDPA_Pet Senior Member Location: US Join Date: Apr 2013 Posts: 222	Can we sequence RNA directly Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now? Or now most sequencing center still use cDNA to do metatranscriptomics.

04-06-2017, 09:59 PM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	I know two technologies for direct transcriptome analysis: 1- nanoString: it can only quantify known transcripts 2- MinIon: the kit currently is offered for early access cDNA still is the most common method.

04-07-2017, 05:00 AM	#3
Bukowski Senior Member Location: UK Join Date: Jan 2010 Posts: 390	http://biorxiv.org/content/early/2016/08/12/068809