RNA Sequencing at doctor's office

fuellen · 02-01-2018, 10:23 PM

Hello!
I'm looking for feasible scenarios that would enable gene expression data analyses at a doctor's office, probably based on a blood sample. It seems that sample processing is too sophisticated to be done there, so samples would have to be frozen and shipped. Question: How long can blood samples, after minimal processing, be stored at -18 degrees Celsius (or at +4 degrees, possibly) so that they may then be sent in bulk (eg on dry ice) to a sequencing facility?
Thanks for any hints!

HESmith · 02-02-2018, 05:31 AM

Expression profiles can change in minutes, so I'd recommend adding a stabilization component (e.g., Qiagen's RNAprotect) immediately after collection. At that point, the samples would be stable for weeks at 4˚ and indefinitely if frozen.

GW_OK · 02-09-2018, 07:43 AM

Is there a reason you can't draw into a PAXgene tube?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Does small RNA sequencing generate comparable reads as regular Solexa sequencing?	Goodluck	Illumina/Solexa	1	08-19-2009 02:12 AM

02-01-2018, 10:23 PM	#1
fuellen Junior Member Location: Germany Join Date: Jan 2018 Posts: 8	RNA Sequencing at doctor's office Hello! I'm looking for feasible scenarios that would enable gene expression data analyses at a doctor's office, probably based on a blood sample. It seems that sample processing is too sophisticated to be done there, so samples would have to be frozen and shipped. Question: How long can blood samples, after minimal processing, be stored at -18 degrees Celsius (or at +4 degrees, possibly) so that they may then be sent in bulk (eg on dry ice) to a sequencing facility? Thanks for any hints!

02-02-2018, 05:31 AM	#2
HESmith Senior Member Location: Bethesda MD Join Date: Oct 2009 Posts: 509	Expression profiles can change in minutes, so I'd recommend adding a stabilization component (e.g., Qiagen's RNAprotect) immediately after collection. At that point, the samples would be stable for weeks at 4˚ and indefinitely if frozen.

02-09-2018, 07:43 AM	#3
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 411	Is there a reason you can't draw into a PAXgene tube?