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Old 03-30-2011, 10:58 PM   #1
Zoe rui
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Location: Beijing

Join Date: Mar 2011
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Question Analysis of the 454 results

I'm fresh in 454 sequencing and here, but I am keen to know more information on this field. Then I have a simple question:
>contig00005 length=47 numreads=179 gene=isogroup00001 status=it_thresh
what's the meaning of the "numreads" and "gene=isogroup00001 "?
Thanks very much.
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Old 03-31-2011, 07:16 AM   #2
Rick Westerman
Location: Purdue University, Indiana, USA

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numreads should be, I hope, obvious. It is simply the number of reads making up the contig.

gene is the large grouping from which the contig arises. Looking at the 454 Newbler manual will give a better feel for the differences between contigs and isogroups -- you really need to know this if you are doing 454 sequencing. Also I and others have written about isogroups in SeqAnswers. Take a search through the forum.
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Old 03-31-2011, 08:51 AM   #3
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Location: Purdue University, West Lafayette, Indiana

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I recommend reading Lex's (flxlex) blog:

In depth information on GS-FLX software.

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Old 04-01-2011, 03:19 AM   #4
Zoe rui
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Thanks so much
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