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07-27-2011, 03:15 AM
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Member
Location: Padova ITALY Join Date: Jul 2009
Posts: 32
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Mate-Pair Illumina library problems
Hi, I am about to start a resequencing project on the HiSeq 1000 searching for rearrangements in a bacterial genome.
This is the 1st time I am going to use the mate pair approach, so I am wondering if any of you can share their experience about the protocol especially concerning crucial steps, tips, tricks, and any particular problem you may have had during the library prep. I had a bad experience with paired end library prep on 454 (8-20kbp) last year so I am concerned about starting this new protocol. Thanks to the folks who will share their experience 
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| Tags |
| illumina, mate-pair, mate-pair reads, trueseq |
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