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08-22-2011, 11:30 AM
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Senior Res.
Location: Plovdiv, Bulgaria Join Date: Oct 2008
Posts: 110
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smallRNA GAIIx raw fastq files - quality filter?
Dear all,
I have raw fastq files and I want to do with the help of FASTX toolkit: 1.fastq to fasta 2. clip adaptors 3.collapse to unique sequences But before that I;m not aware what quality filter to apply to the fastq file in order to remove not quality entries/sequences (probably with fastq_quality_filter sub-program)? Or if not FASTX do you know what tool to use to make quality filter run and the default option for smallRNA-seq? Thanks!!! |
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