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Old 04-12-2012, 12:43 PM   #1
polymerase1986
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Location: USA

Join Date: Feb 2012
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Default Library quality check before capturing

Dear all

To check the quality of the genomic DNA library, our collaborators performed a Titration run (1x50) on HiSeq and provided us 50 fasta sequences plus 100'000 fast-q sequences for each library.
Does this mean, that they loaded the created labrary (prior the exome capturing step) on a flow cell? or how does this work? Does anyone have experience with that? What does titration run mean?

Is the quality of the library at this stage - prior to the exome capturing - comparable with the quality of the library after the capturing step?

Thanks a lot for your answers!!

Last edited by polymerase1986; 04-12-2012 at 12:52 PM.
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