Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
ChIP seq - Different DNA size on agarosgel and Bioanalyzer Leeenaaa Sample Prep / Library Generation 73 11-02-2014 08:45 AM
Ideas on Bioanalyzer trace for ChIP-seq library? orlatron Sample Prep / Library Generation 0 05-14-2012 11:02 PM
ChIP-Seq: Whole-genome chromatin profiling from limited numbers of cells using nano-C Newsbot! Literature Watch 0 10-01-2011 03:30 AM
ChIP seq - Different DNA size on agarosgel and Bioanalyzer Leeenaaa Introductions 0 02-21-2011 01:23 AM
Yeast ChIP-seq; Sonication/Bioanalyzer question AaronS Sample Prep / Library Generation 1 01-14-2011 07:55 AM

Thread Tools
Old 06-13-2012, 09:58 PM   #1
Junior Member
Location: India

Join Date: Aug 2011
Posts: 1
Angry RNA Nano bioanalyzer chip

I am using RNA Nano bioanalyzer chip to assess the quality of the total RNA after isolation using Trizol and purification using Qiagen column. Unfortunately all the peaks in BA graphs look shifted i.e. they are late migrating. I am using the BA system which is just one year old and all the diagnostic tests look fine. What could be the possible reason for this?
Attached Images
File Type: png Screenshot-42.png (85.7 KB, 37 views)
dkunal is offline   Reply With Quote
Old 06-14-2012, 05:30 AM   #2
Senior Member
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

I posted a fix for this (fairly common) issue previously here.

See if that will work for you.

pmiguel is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:49 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO