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Old 04-27-2013, 06:44 AM   #1
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Location: Brazil

Join Date: Apr 2011
Posts: 3
Default Sequencing 400bp 16s Amplicons on the PGM

Dear all,

I would like to know if anyone has tried sequencing 16s rDNA amplicons that are in the correct size range (~320bp) using the Ion Plus Fragment Library Kit and One Touch System 2 then sequenced them using the new 400bp chemistry on a 314 v2 chip. Are there any incompatibilities? How does the data look? Even if they are not 16s amplicons, but other amplicons, I'm interested in your feedback.

Thanks in advance,

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