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03-28-2010, 11:57 AM
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#1 |
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Junior Member
Location: Oregon Join Date: Mar 2010
Posts: 2
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Can you really see miRNAs on gels before PCR amplification
I am new to miRNAseq. We plan to do miRNAseq using small RNA prep V1.0 and I am wondering if we could really see miRNAs on gel after each ligation?
Thanks a lot. |
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07-21-2010, 09:24 AM
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#2 |
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Senior Member
Location: Boston,MA Join Date: Nov 2008
Posts: 122
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miRNA
I have never been able to see them. I just do a blind cut and pray a lot!
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07-21-2010, 09:44 AM
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#3 | |
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Junior Member
Location: Oregon Join Date: Mar 2010
Posts: 2
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Thank you!
I finally cut it blindly too. But it worked! Quote:
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07-27-2010, 07:37 PM
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#4 |
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Member
Location: US Join Date: May 2010
Posts: 21
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if you use an adenylated 3' adapter you don't have to perform the small RNA gels and cuts. the adapter specifically will bind to your smalls.
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