Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Total RNA quality (Bioanalyzer trace attached) peromhc Sample Prep / Library Generation 26 10-12-2015 04:36 PM
Bioanalyzers: High 28S rRNA Peak libprep Sample Prep / Library Generation 6 12-20-2012 04:30 AM
Bioanalyzer expired RNA Nano 6000 kit arkal Sample Prep / Library Generation 3 10-09-2012 12:36 AM
odd total RNA pattern bioanalyzer eab Sample Prep / Library Generation 18 08-18-2012 05:54 AM
RNA Nano bioanalyzer chip dkunal Illumina/Solexa 1 06-14-2012 05:30 AM

Thread Tools
Old 12-02-2016, 06:42 AM   #1
Junior Member
Location: Boston, MA

Join Date: Dec 2016
Posts: 2
Default Bioanalyzer RNA Nano with total RNA - High MW peak above 28S?

I'm getting a significant high MW peak on a nano RNA chip with total RNA. Any thoughts on what this might be caused by? It is present in all the total RNA samples I loaded on this chip.

dlv04c is offline   Reply With Quote
Old 12-06-2016, 11:39 PM   #2
Junior Member
Location: Australia

Join Date: Nov 2016
Posts: 2

Possibly genomic DNA. Have you digested your DNA and if so with what?

Some good info here:
Amar is offline   Reply With Quote
Old 12-07-2016, 10:40 AM   #3
Junior Member
Location: Boston, MA

Join Date: Dec 2016
Posts: 2
Default it was not denatured

Turns out the RNA was not denatured prior to loading on the chip.
dlv04c is offline   Reply With Quote
Old 10-24-2017, 07:38 AM   #4
Junior Member
Location: Germany

Join Date: Feb 2017
Posts: 1

Did it help? You don't see this peak anymore?
I also have problem with this peak (though it is a bit smaller in my case). I am always denaturing my RNA prior to the run but maybe 2 min is not enough?

I think it is not DNA as second DNase treatment did not help and qPCR (+RT vs -RT, housekeeping gene) shows that the RNA is free of DNA (no amplification after 40 cycles). I also tried spiking in a small amount of gDNA and it never resulted in such electropherogram.

Then again I have seen this peak in many publications and examples of "good RNA". fig 7.9 fig 1.

What can that be?
I am very puzzled.
Attached Images
File Type: png Screen Shot 2017-10-24 at 16.29.00.png (141.0 KB, 11 views)
marta.m_ is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:46 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO