Flow cell

Kultamuna2 · 03-11-2018, 07:53 AM

Hi! Is the flow cell analyzed in one go per base (as a whole), or scanned one part of the flow cell at a time, until all bases have been identified? How many cameras are there in llumina's instruments to image the flow cell? Thanks in advance!

JoeKutch · 03-12-2018, 07:41 AM

As far as I understand, the MiSeq, HiSeq 2500, and HiSeq 4000 each have a single camera. The flow cell is divided into lanes, swaths (left/right/center depending on the machine), surfaces (top and bottom), and tiles. A single tile is imaged at a time, except during focusing. The camera moves up a swath, imaging each tile's top surface. It does all the swaths for the top surface, then goes back and does the bottom surface for each tile.

The NextSeq is slightly different, with six cameras/sensors working as a single module, simultaneously imaging six different tiles.

I can't speak to the NovaSeq or iSeq.

Kultamuna2 · 03-12-2018, 05:26 PM

Thanks a lot for the good answer, it helped a lot!

GW_OK · 03-13-2018, 07:11 AM

I do know Hiseq 2500's have two cameras. Here's a picture of the optical deck in case anyone is interested. You can see the green and red lasers at the left and top and the two cameras at angles on the right.

Click for bigger version.

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03-11-2018, 07:53 AM	#1
Kultamuna2 Junior Member Location: Austria Join Date: Mar 2018 Posts: 5	Flow cell Hi! Is the flow cell analyzed in one go per base (as a whole), or scanned one part of the flow cell at a time, until all bases have been identified? How many cameras are there in llumina's instruments to image the flow cell? Thanks in advance!

03-12-2018, 05:26 PM	#3
Kultamuna2 Junior Member Location: Austria Join Date: Mar 2018 Posts: 5	Thanks Thanks a lot for the good answer, it helped a lot!

03-12-2018, 07:41 AM	#2
JoeKutch Member Location: Philadelphia Join Date: Oct 2016 Posts: 17	As far as I understand, the MiSeq, HiSeq 2500, and HiSeq 4000 each have a single camera. The flow cell is divided into lanes, swaths (left/right/center depending on the machine), surfaces (top and bottom), and tiles. A single tile is imaged at a time, except during focusing. The camera moves up a swath, imaging each tile's top surface. It does all the swaths for the top surface, then goes back and does the bottom surface for each tile. The NextSeq is slightly different, with six cameras/sensors working as a single module, simultaneously imaging six different tiles. I can't speak to the NovaSeq or iSeq.

03-13-2018, 07:11 AM	#4
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 411	I do know Hiseq 2500's have two cameras. Here's a picture of the optical deck in case anyone is interested. You can see the green and red lasers at the left and top and the two cameras at angles on the right. Click for bigger version.